|
KMID : 0894520070110010049
|
|
Development & Reproduction
2007 Volume.11 No. 1 p.49 ~ p.54
|
|
|
Effect of Ethane 1,2-Dimethane Sulfonate(EDS) on the Expression of Pituitary Gonadotropin in Male Rats
|
|
Son Hyeok-Jun
Kim Soo-Woong
Paick Jae-Seung
Lee Sung-Ho
|
|
|
Abstract
|
|
|
|
Ethane 1,2-dimethane sulfonate(EDS), a toxin which specifically kills Leydig cells(LC), has been widely used to prepare the reversible testosterone(T) depletion rat model. In the present study, we monitored the gene expression profiles of pituitary gonadotropins, LH and FSH, up to 7 weeks after EDS injection. Adult male Sprague-Dawley rats(300～350 g B.W.) were injected with a single dose of EDS(75 ㎎/㎏ i.p.) and sacrificed on weeks 0, 1, 2, 3, 4, 5, 6 and 7. Total RNAs were purified from each pituitary, and the message levels of common alpha subunit(Cα) of pituitary glycoprotein hormones, LH beta subunit(LHβ), FSH beta subunit(FSHβ) and GnRH receptor(GnRH-R) were evaluated by semi-quantitative RT-PCRs. The message levels of Cα increased sharply during weeks 1-4, then return to the control level on week 5. The mRNA levels of LHβ were elevated after week 2, reached the peak at week 4, then declined to the control level after week 5. The message levels of FSHβ were elevated after week 2, reached the peak at week 3, then declined to the nadir at week 5. Similarly, the mRNA levels of GnRH-R were elevated after week 2, reached the peak at week 3, then gradually declined to the control level after week 5. The present study indicated that EDS treatment could induce reversible alterations in the transcriptional activities of gonadotropin subunits and GnRH-R in the anterior pituitary from male rats. EDS injection model might be useful to understand the mechanism of hormonal regulation of hypothalamus- pituitary neuroendocrine axis in male rats.
|
|
|
KEYWORD
|
|
|
Ethane Dimethane Sulfonate(EDS), Pituitary, Gonadotropin subunits, Expression profiles
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|